Journal: Fluids and Barriers of the CNS

Article Title: Accurate, strong, and stable reporting of choroid plexus epithelial cells in transgenic mice using a human transthyretin BAC

doi: 10.1186/s12987-018-0107-4

Figure Lengend Snippet: TdTomato continues to be expressed in postnatal choroid plexus of BAC transgenic mice. A Show paired images of transmitted light and tdTomato fluorescence in wet mounts of dissected lateral ventricle ChP from post-weanling mice from three founder lines. Whereas transgenic ChP (+) fluoresce brightly, ChP from wild-type siblings (−) do not. B Show immunofluorescence in cryostat sections from a P3 brain. TdTomato was detected in all four ventricles and was co-localized with anion exchanger 2 (AE2, right). C – E Shows immunofluorescence in microtome sections of brains from 314-day-old mice, verifying the long-term expression of the reporter in CPECs of the lateral ( C ), third ( D ), and fourth ( E ) ventricle. The selective localization of tdTomato in CPECs is confirmed by associated staining with antibodies to TTR. The location of nuclei is indicated by DAPI staining

Article Snippet: After three additional washes, incubations with secondary antibodies (1:500) were carried out for 1 h at room temperature, followed by three additional washes and mounting with Fluoromount-G containing DAPI fluorescent counterstain (SouthernBiotech, Birmingham, AL).

Techniques: Transgenic Assay, Fluorescence, Immunofluorescence, Expressing, Staining